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. 2022 Jun 1;13(2):71. doi: 10.3390/jfb13020071

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vitro culture of the dental epithelial cell line HAT-7 in self-crosslinked oxidized alginate-carboxymethyl chitosan hydrogels with three weight ratios of 2:1, 3:1, and 4:1 for 14 days. (A) Observation of cell morphology in cell-laden hydrogels using optical phase contrast microscopy. (B) Alkaline phosphatase and (C) Alizarin red S staining (markers of maturation stage ameloblasts) of HAT-7 cells in self-crosslinked hydrogels, showed initiation of mineralization and ameloblast differentiation.