Table 1.

Metabolic reaction equations and metabolic node reaction rate equations of ganoderic acids R, S and T’s synthesis from submerged fermentation-static culture of G. lucidum.

Metabolic Reaction Equations	Metabolic Node Reaction Rate Equations
Glycolysis pathway (EMP):	(1) GLC: GLC(e) − r1 = 0
r1: GLC + ATP → G6P + ADP	(2) G6P: r1 − r2 − r10 = 0
r2: G6P → F6P	(3) F6P: r2 − r3 + r13 + r14 = 0
r3: F6P + ATP → 2GA3P + ADP	(4) GA3P: 2r3 − r4 + r14 = 0
r4: GA3P + NAD + PI + 2ADP → PYR + NADH + 2ATP	(5) PYR: r4 − r5 − r6 = 0
Tricarboxylic acid cycle (TCA):	(6) AcCoA: r5 − r7 − 2r15 − r16 = 0
r5: PYR + CoA + NAD → AcCoA + NADH + CO2	(7) ICI: r7 − r8 = 0
r6: PYR + ATP + CO2 → OAA + ADP + PI	(8) α-KG: r8 − r9 = 0
r7: OAA + AcCoA → ICI + CoA	(9) OAA: r6 − r7 + r9 = 0
r8: ICI + NADP → α − KG + NADPH + CO2	(10) Ru5P: r10 − r11 − r12 = 0
r9: α − KG + 2NAD + FAD + ADP + PI → OAA + 2NADH + FADH2 + ATP + CO2	(11) X5P: r11 − r13 − r14 = 0
Pentose phosphate pathway (PP):	(12) R5P: r12 − r13 = 0
r10: G6P + 2NADP → Ru5P + 2NADPH + CO2	(13) E4P: r13 − r14 = 0
r11: Ru5P → X5P	(14) CoA: -r5 + r7 + r15 + 2r16 = 0
r12: Ru5P → R5P	(15) AcAcCoA: r15 − r16 = 0
r13: X5P + R5P → F6P + E4P	(16) IPP: r16 − r17 − r18 − r19 − r20 = 0
r14: X5P + E4P → F6P + GA3P	(17) DMAPP: r17 − r18 = 0
Ganoderic acids R, S, T synthesis pathway (GAP):	(18) GPP: r18 − r19 = 0
r15: 2AcCoA → AcAcCoA + CoA	(19) FPP: r19 − r20 − 2r21 = 0
r16: AcAcCoA + AcCoA + 2NADPH + 2H+ + 3ATP + H2O → IPP + 2CoA + 2NADP+ + 3ADP + PI + CO2	(20) SQ: r21 − r22 = 0
r17: IPP → DMAPP	(21) OS: r22 − r23 = 0
r18: DMAPP + IPP → GPP + PPI	(22) LAN: r23 − r24 − r25 − r26 = 0
r19: GPP + IPP → FPP + PPI	(23) ERG: r24 − ERG(e) = 0
r20: FPP + IPP → GGPP + PPI	(24) GaR: r26 − GaR(e) = 0
r21: 2FPP + NADPH + H+ → SQ + NADP+ + 2PPI	GaS: r26 − GaS(e) = 0
r22: SQ + O2 + NADPH + H+ → OS + NADP+ + H2O	GaT: r26 − GaT(e) = 0
r23: OS → LAN
r24: LAN → ERG
r25: LAN → MY
r26: LAN → GaR/GaS/GaT