KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
|---|---|---|---|---|
| Antibodies | ||||
| Chicken anti-GFP | Aves Labs | Cat# GFP-1020, RRID:AB_10000240 | ||
| Rabbit anti-TH | EMD Millipore | Cat#AB152, RRID:AB_390204 | ||
| Rat anti-mCherry mAb | ThermoFisher | Cat#M11217, RRID:AB_2536611 | ||
| Donkey anti-chicken AlexaFluor488 | Jackson ImmunoResearch | Cat# 703-545-155, RRID:AB_2340375 | ||
| Donkey anti-rat Cy3 | Jackson ImmunoResearch | Cat# 712-165-153, RRID:AB_2340667 | ||
| Donkey anti-rabbit AlexaFluor 647 | Molecular Probes | Cat# A-31573, RRID:AB_2536183 | ||
| Bacterial and virus strains | ||||
| AAV5-CAG-FLExFRT-TC | University of North Carolina, vector core | N/A | ||
| AAV8-CAG-FLExFRT-RABV-G | University of North Carolina, vector core | N/A | ||
| AAVDJ-hSyn-FLExFRT-hM4Di | University of North Carolina, vector core | N/A | ||
| AAVDJ-hSyn-FLExFRT-hM3Dq | Gene Vector and Virus Core lab at Stanford University | N/A | ||
| AAV DJ EF1 α-fDIO-eYFP | University of North Carolina, vector core | N/A | ||
| AAV5-EF1 α-fDIO-GCaMP6f | Gene Vector and Virus Core lab at Stanford University | N/A | ||
| AAVretro-EF1 α-fDIO-Cre | Addgene | Cat121675-AAVrg | ||
| AAV1hSyn-FLExloxP-jGCaMP7f | Addgene | Cat#104488-AAV1 | ||
| AAV8-hSyn-FLExFRT-mGFP-2A-Synaptophysin-mRuby | University of North Carolina, vector core | N/A | ||
| CAV-FLExloxP-Flp | Plateforme de Vectorologie de Montpellier, France | N/A | ||
| RABV▵DG | This paper | N/A | ||
| Chemicals, peptides, and recombinant proteins | ||||
| Cocaine hydrochloride | Sigma | Cat#C5776-1G; CAS:53-21-4 | ||
| Morphine | Patterson Veterinary | Cat#78924699 | ||
| Clozapine-N-oxide (CNO) | Hello bio | Cat#HB1807 | ||
| Degradex PLGA CNO microspheres | Phosphorex | Cat#LG1000-CNO | ||
| 2,4,5 trimethylthiazoline (TMT) | BioSRQ | Cat#1G-TMT-90 | ||
| Paraformaldehyde | Sigma | Cat#P6148; CAS: 30525-89-4 | ||
| Triethanolamine | Sigma | Cat#T58300; CAS: 102-71-6 | ||
| Acetic anhydride | Sigma | Cat#242845; CAS: 108-24-7 | ||
| Formamide | Sigma | Cat#47671-1L-F; CAS: 75-12-7 | ||
| Dextran sulfate | Sigma | Cat#D8906; CAS: 9011-18-1 | ||
| Tween-20 | Sigma | Cat#P9416; CAS: 9005-64-5 | ||
| Deposited data | ||||
| Fiber photometry recording | This paper | Zenodo: https://doi.org/10.5281/zenodo.6415883 | ||
| RABV tracing data | This paper | Zenodo: https://doi.org/10.5281/zenodo.6415883 | ||
| Experimental models: Organisms/strains | ||||
| Mouse B6.SJL-Slc6a3tm1.1(cre)Bkmn/J (DAT-Cre) | The Jackson laboratory | RRID:IMSR_JAX:006660 | ||
| Mouse Slc32a1-2A-FlpO-D knock-in (vGAT-Flp) | The Jackson laboratory | RRID:IMSR_JAX:029591 | ||
| Oligonucleotides | ||||
| See Table S1 for the list of probes for FISH | This paper | N//A | ||
| Clean G | This paper | N/A | ||
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
| Oligo imager | This paper | N/A | ||
| 42 bp hairpin sequence | This paper | N/A | ||
| Software and algorithms | ||||
| Imaris | Bitplane | N/A | ||
| MATLAB | MathWorks Inc | N/A | ||
| Prism 9 | GraphPad https://www.graphpad.com/scientific-software/prism/ | N/A | ||
| Fiji | https://imagej.net/software/fiji/?Downloads | N/A | ||
| Biobserve | http://www.biobserve.com/ | N/A | ||
| Python 3.8 | https://www.python.org/downloads/release/python-380/ | N/A | ||
| MATLAB script for FP analysis | This paper | Zenodo: https://doi.org/10.5281/zenodo.6415661 | ||
| MATLAB script for PSTH analysis | This paper | Zenodo: https://doi.org/10.5281/zenodo.6415661 | ||
| Python codes for PSTH analysis | This paper | Zenodo: https://doi.org/10.5281/zenodo.6415661 | ||
| Other | ||||
| Superfrost Plus Micro slides | VWR | Cat#48311-703 | ||
| Fluoromount-G™ | Invitrogen | Cat#00-4958-02 | ||
| Cover glass | Thermo Scientific | Cat#152460 | ||
| Optical fiber | THORLABS | Cat#FT400EMT | ||
| Vetbond tissue adhesive | Patterson Veterinary | Cat#78055031 | ||
| Vetameg | Patterson Veterinary | Cat# 07-892-4375 | ||
| Metal screws | Antrin Miniature Specialists | Cat#AMS90/1B | ||
| Metabond | Parkell | Cat#S380 | ||
| Geristore A | Den-Mat holdings | Cat#4506 | ||
| Geristore B | Den-Mat holdings | Cat#034523101 | ||