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. 2000 Sep;66(9):4029–4036. doi: 10.1128/aem.66.9.4029-4036.2000

TABLE 1.

Primers and fluorogenic probe for specific detection of C. jejuni

Probe or primer Sequence (5′ - 3′) Denaturation temp (°C)a
  Primers
   Forward CTG AAT TTG ATA CCT TAA GTG CAG C 60.4
   Reverse AGG CAC GCC TAA ACC TAT AGC T 60.3
  Probe TCT CCT TGC TCA TCT TTA GGA TAA ATT CTT TCA CA 66.6
a

Calculated by the nearest-neighbor algorithm with the Primer Express program (primer concentration, 300 nM; probe concentration, 20 nM; salt concentration, 55 nM).