TABLE 1.
Probe or primer | Sequence (5′ - 3′) | Denaturation temp (°C)a |
---|---|---|
Primers | ||
Forward | CTG AAT TTG ATA CCT TAA GTG CAG C | 60.4 |
Reverse | AGG CAC GCC TAA ACC TAT AGC T | 60.3 |
Probe | TCT CCT TGC TCA TCT TTA GGA TAA ATT CTT TCA CA | 66.6 |
Calculated by the nearest-neighbor algorithm with the Primer Express program (primer concentration, 300 nM; probe concentration, 20 nM; salt concentration, 55 nM).