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. 2021 Oct 31;18(6):1401–1415. doi: 10.1080/15548627.2021.1987671

Figure 3.

Figure 3.

The induction of mitophagy enhances the intracellular survival of M. bovis. (A and B) J774A.1 and BMDM were treated with DMSO (0.2%), CCCP (10 μM) or Mdivi-1 (20 μM) for 2 h and then infected with M. bovis at MOI of 10 for 24 h. Intracellular M. bovis load were measured by colony-forming units (CFU). (C) BMDM were treated with DMSO (0.2%), CCCP (10 μM) or Mdivi-1 (20 μM) for 2 h, then infected with M. bovis (MOI = 10). Intracellular M. bovis load was analyzed by confocal microscopy at 0 h and 24 h post-infection. (D) The ratio of bacteria number to cells number. One hundred cells in 10 fields were counted in each group. (E and F) J774A.1 and BMDM were transfected with Pink1 siRNA (25 nM) and negative control siRNA (25 nM) for 48 h and then infected with M. bovis (MOI = 10). Intracellular M. bovis load were measured by CFU at 0 h and 24 h post-infection. (G) Schematic illustration of the results of Figure 3. Data shown as means ± SEM from three independent experiments. Statistical analysis was done by using Unpaired t-test (two-tailed). *p < 0.05, **p < 0.01, ns, not significant.