Figure 10.

Triggering the PRKAA1-dependent autophagy-mediated proteolysis with AICAR in ART improves their cellular rates of mitochondrial β-oxidation and Mem-related IL21 production. (A-C) We polyclonally activated Mem from ART in the presence or absence of PRKAA1-dependent autophagy-mediated proteolysis (AICAR with or without BaF, and E64d-PepA) induction, before assessing their mitochondrial respiration. (A) Representative respiratory kinetics of Mem from ART at 6 h post-polyclonal activation when cells have been treated with or without PRKAA1-dependent autophagy-mediated proteolysis. OCR, oxygen consumption rate. (B) SRC and (C) ATP-linked respiration were determined for all culture conditions. (D) At 6 h of polyclonal and HIV-1-specific activation, we also monitored the intracellular levels of IL21 with or without AICAR and E64d-PepA treatments. N = 6. The error bars indicate standard deviations from the means. β, symbol used for paired t-test (comparison between treated Mem and untreated control). *, symbol used for Mann-Whitney test (comparison between study groups).