Table 1.

Antibodies used

	Multi-parameter antibodie coktails developed.
	a. For the autophagy assessment
	Immunogen	clone ID	fluorophore	Catalog number	vendor
Surface	CD3W	UCHT-1	Alexa Fluor 700	557943	BD Biosciences
	CD4	RPA-T8	V450	560346	BD Biosciences
	CD45RA	RPA-T4	APC H7	561212	BD Biosciences
IC	ULK1	F-4	PE	sc-390904	Santa Cruz Biotechnology
	BECN1	EPR 20473	Alexa Fluor 647	ab246760	Abcam
	ATG7	B-9	Alexa 488	sc-376212	Santa Cruz Biotechnology
	Immunogen	clone ID	fluorophore	Catalog number	vendor
Surface	CD3W	UCHT-1	Alexa Fluor 700	557943	BD Biosciences
	CD4	RPA-T8	V450	560346	BD Biosciences
	CD45RA	RPA-T4	APC H7	561212	BD Biosciences
IC	SQSTM1	5F2	Alexa Fluor 488	M162-A48	MBL Life Sciences
	b. For MTOR assessment
(i)	Immunogen	clone ID	fluorophore	Catalog number	vendor
Surface	CD3W	UCHT-1	Alexa Fluor 700	557943	BD Biosciences
	CD4	RPA-T8	V450	560346	BD Biosciences
	CD45RA	RPA-T4	APC H7	561212	BD Biosciences
IC	MTOR p-S2448	MRRBY	PE	215Q18	Invitrogen (ThermoFisher)
	Total MTOR	EPR 20473	unconjugated*	12–9718-42	Invitrogen (ThermoFisher)
	*Abs were conjugated to AlexaFluor 647dye using the Zenon mouse IgG1 labeling kit (ThermoFisher Scientific, Z25008)
(ii)	Immunogen	clone ID	fluorophore	Catalog number	vendor
Surface	CD3W	UCHT-1	Alexa Fluor 700	557943	BD Biosciences
	CD4	RPA-T8	V450	560346	BD Biosciences
	CD45RA	RPA-T4	APC H7	561212	BD Biosciences
IC	EIF4BP1 p-Thr36/45	V3NTY24	PE	12–9107-42	Invitrogen (ThermoFisher)
	c. For the immune function assessment
	Immunogen	clone ID	fluorophore	Catalog number	vendor
Surface	CD3W	UCHT-1	Alexa Fluor 700	557943	BD Biosciences
	CD4	RPA-T8	V450	560346	BD Biosciences
	CD45RA	RPA-T4	APC H7	561212	BD Biosciences
IC	IFNG	4S.B3	Alexa Fluor 647	563495	BD Biosciences
	IL21	3A3-N2	PE	560463	BD Biosciences