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. 2022 Jun 14;8(6):632. doi: 10.3390/jof8060632

Table 2.

Beneficial bacteria which demonstrated protection against main wheat pathogens. n/a: not applicable.

Strains Origin Pathogen Biostimulation Mode of Action for Biocontrol Methodology References
Bacillus subtilis ATCC 10783; B. cereus ATCC 11778; B. licheniformis NRRLB-510; B. pumilus ATCC 7061; Brevibacillus laterosporus BLA170; Paenibacillus polymyxa NA Soil Zymoseptoria tritici, Pyrenophora tritici-repentis, Cochliobolus sativus, Alternaria triticimaculans n/a n/a In vitro and greenhouse (leaves) [121]
B. subtilis BBG125, BBG131, Bs2504 ProBioGEM, Centre Wallon de Biologie Industrielle Z. tritici n/a Lipopeptides (LPs): mycosubtilin, surfactin, fengycin In vitro and greenhouse (leaves) [122]
B. velezensis RC 218 Wheat anthers Fusarium graminearum n/a Ericin lantiobiotic
Plant phytohormone modulation (jasmonic and salicylic acid)
ISR cell wall thickening preventing cell plasmolysis and collapse
Field and greenhouse (spikes) [123,124,125,126]
B. velezensis LM2303 Wild yak F. graminearum n/a n/a In silico and field (spikes) [127]
B. subtilis IB Soil F. graminearum n/a Fengycin In vitro [128]
B. megaterium BM1 and B. subtilis BS43, BSM0, BSM2 Wheat spikes F. graminearum n/a Degradation of DON Metabolites In vitro and field (spikes) [129]
B. subtilis BaSu1/BaSu3, B. amyloliquefaciens BaAm and Chaetomium globosum CG1/CG2, Phoma glomerata PG1, Aureobasidium proteae AP5 and Sarocladium kiliense SK1/SK2 Wheat endosphere F. graminearum and F. culmorum n/a Antibiosis In vitro and greenhouse (detached wheat spikelets) [22]
B. amyloliquefaciens TrigoCor Wheat rhizosphere F. graminearum n/a Iturin Field and greenhouse (spikes) [130]
B. amyloliquefaciens S76-3 Wheat spikes F. graminearum n/a Iturin A and plipastatin In vitro [131]
B. amyloliquefaciens Y1 Soil F. graminearum n/a Metabolites Cyclo D-PRO-L- VAL In vitro [132]
B. amyloliquefaciens B8 and B3 Soil F. graminearum and culmorum Yes Phytohormones In vitro and greenhouse [133]
B. amyloliquefaciens BLB369, B. subtilis BLB277, Pae. polymyxa BLB267 Soil F. graminearum Yes Supernatant (iturin and surfactin, fengycin, fusaricidin and polymyxin) In vitro and greenhouse [134]
B. megaterium MKB135
Pseudomonas fluorescens MKB21 and MKB91
Cereal rhizospheres, leaves, grain and weeds Z. tritici Yes Cell free surpernantant and VOC Field and greenhouse (leaves) [135]
B. subtilis strains 53 and 71, P. fluorescens biov1 strain 32 and Streptomyces sp. strain 3 Wheat kernels F. graminearum Yes Volatiles metabolites In vitro and greenhouse (spikes) [136]
Antibiotics tubercidin, phosphlactomycin and candicidin, 2,4-diacetylphloroglucinol, phenasin, fengymcine, bacillomycin
Phytohormone regulation
B. subtilis RC 218, S. sp. BRC87B. and Brevibacillus sp. BRC263 Wheat anthers F. graminearum Yes Space and nutrients competition Field, greenhouse and in vitro [125,126,137,138,139,140]
Metabolites
Streptomyces. sp. DEF09 Wheat root F. graminearum Yes Metabolites Field, greenhouse (spikes) and in vitro [141]
IAA
Streptomyces. sp. BN1 Rice kernels F. graminearum n/a n/a In vitro and greenhouse (seeds and spikes) [142]
B. cereus Soil from wheat fields F. graminearum Yes Dose and cultivar dependent In vitro and greenhouse (seeds) [143]
B. subtilis AS43.3/AS43.4, Cryptococcus sp. OH71.4 and Cryptococcus nodaensis OH182.9 Wheat anthers F. graminearum n/a n/a Field, greenhouse (spikes) and in vitro [144,145]
Co-cultures of B. subtilis OH 131.1 and Cryptococcus flavescens OH 182.9 ARS NRRL F. graminearum n/a Plipastatin and subtilomycin Greenhouse (spikes) [146]
Lactobacillus brevis JJ2P; Lactobacillus reuteri R2 Porcine gut, cheese Z. tritici n/a Cell free supernatant (phenyllactic acid and hydroxyphenyllactic acid) In vitro and greenhouse (leaves) [147]
Lactobacillus plantarum strain 21B Sourdough breads F. graminearum n/a Antifungal phenyllactic acid and 4-hydroxyphenyllactic acid In vitro [148]
Paenibacillus polymyxa SGK2 Wheat rhizosphere F. graminearum, F. culmorum, F. verticillioides Yes Competition for nutrients (iron) In vitro and greenhouse (seeds) [149]
Pae. polymyxa W1-14-3 and C1-8-b Rhizosphere F. graminearum Yes Inhibition of fungal germination In vitro and greenhouse (spikes) [150]
glucanolytic enzyme, cellulase, mannanase xylase, chitinase and protease
supernatant activity (enzymatic or antibiotic activities: polymyxins, benzoic acid, fusaricidin A and antibiotic peptides)
Pae. sp. B2 INRAE Dijon Z. tritici n/a ISR Field and greenhouse (leaves) [151]
P. fluorescens LEC1 Wheat phyllosphere Z. tritici n/a Antibiotics 1- hydroxyphenazine and chlororaphin In vitro and greenhouse (leaves) [152]
P. fluorescens PFM2 Wheat phyllosphere M. graminicola n/a Antibiotics 2-4-diacetylphoroglucinol and phenazine-l-carboxylic acid In vitro [153]
P sp. AS 64.4 Wheat anthers F. graminearum n/a Nutrients competition (choline metabolizing strain) Field, greenhouse (spikes) and in vitro [154]
P. putida BK8661 Wheat phyllosphere Z. tritici Yes HCN, siderophore, antibiotics In vitro and greenhouse (leaves) [155]
P. aeruginosa LEC1 Soil Z. tritici n/a Antibiotic (Pyocyanine) and Siderophore (pyoverdine) In vitro and field (leaves) [156]
P. chlororaphis MA 342 Craw berry rhizosphere Septoria nodorum n/a n/a Field (seeds) [157]
P. piscium ZJU60 Wheat anthers F. graminearum n/a Phenazine-1-carboxamide Field and greenhouse (spikes) [158]
P. fluorescens LY1-8 Wheat tissues F. graminearum n/a Extracellular hydrolytic enzymes (protease, chitinase, cellulose, glucanase and siderophore) and antagonistic activity Field and greenhouse (spikes) [159]
Devosia sp. strain NKJ1 and Nocardioides spp. strains SS3 or SS4 Wheat field soil F. graminearum Yes Degradation of DON In vitro [160]

Rows highlighted in grey depict the authors hypothesis for the mode of action of beneficial bacteria based on their preliminary studies.