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. 2022 Mar 29;18(5):2048622. doi: 10.1080/21645515.2022.2048622

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Figure 2. — Quantification of hACE2 protein in HIS-DRAGA lungs.

Lung lysates were immunoprecipitated using S1 (RBD)-mFcγ2a protein + rat anti-mouse IgG2a-magnetic beads, and eluted hACE2 levels were quantified by ELISA (upper panel), as described in Materials and Methods. Inserted image shows immunoblot analysis of hACE2 immunoprecipitated from human lungs (lane 1), hACE2 immunoprecipitated from HIS-DRAGA mouse lungs (lane 2), and the absence of hACE2 in the lung immunoprecipitate from lungs of a non-HIS-reconstituted DRAGA mice (lane 3). Lower panel provides the data used to calculate the amount of hACE2 immunoprecipitated from human lungs as compared to HIS-DRAGA mouse lungs.