Table 1.
Human immune parameters of HIS-DRAGA mice
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Peripheral blood |
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| Mouse* | Gender | Time post-infusion with human stem cells | % human B cells (CD19+) | % human T cells (CD3+) |
SARS-CoV-2 challenge dose (pfu) | Euthanasia (days post-infection) | Cord blood HLA haplotype^ | Cord blood gender |
| M#1 | M | 21 weeks | 4.5 | 13.5 | 104 | 1 | A | M |
| F#1 | F | 21 weeks | 48.6 | 9.3 | 104 | 14 | A | M |
| F#2 | F | 21 weeks | 21 | 33.4 | 2.8x103 | 14 | A | M |
| F#3 | F | 24 weeks | 16.1 | 37.8 | 1x103 | 25 | C | M |
| F#4 | F | 16 weeks | 9.0 | 2.5 | 1x103 | 25 | B | F |
| F#5 | F | 24 weeks | 6.0 | 23.8 | 1x103 | 25 | C | M |
| F#6 | F | 30 weeks | 5.9 | 42.6 | 1x103 | 25 | A | M |
| F#7 | F | 16 weeks | 23.6 | 5.0 | 1x103 | 25 | B | F |
| F#8 | F | 16 weeks | 0.6 | 12.5 | 1x103 | 25 | B | F |
| F#9 | F | 16 weeks | 21.5 | 8.0 | 1x103 | 25 | B | F |
| F#10 | F | 16 weeks | 37.0 | 4.7 | 1x103 | 25 | B | F |
| M#2 | M | 30 weeks | 8.3 | 9.3 | 1x103 | 4 | A | M |
| M#3 | M | 16 weeks | 5.7 | 15.0 | 1x103 | 4 | B | F |
| M#4 | M | 16 weeks | 2.0 | 51.1 | 1x103 | 4 | B | F |
| F#11 | F | 16 weeks | 6.1 | 25.4 | 1x103 | 4 | B | F |
| F#12 | F | 24 weeks | 7.0 | 8.8 | 1x103 | 4 | C | M |
| F#13 | F | 24 weeks | 0.6 | 26.8 | 1x103 | 4 | C | M |
| a | M | 20 weeks | 18.9 | 28.3 | —- | —- | D | F |
| b | M | 20 weeks | 0.9 | 41.4 | —- | —- | D | F |
| c | F | 24 weeks | 52.5 | 17.5 | —- | —- | D | F |
| d | M | 24 weeks | 7.4 | 16.6 | —- | —- | D | F |
| e | M | 24 weeks | 1.0 | 22.1 | —- | —- | D | F |
| f | M | 24 weeks | 1.2 | 44.7 | —- | —- | D | F |
| g | M | 24 weeks | 17.6 | 38.3 | —- | —- | D | F |
| h | F | 20 weeks | 35.2 | 5.0 | —- | —- | E | M |
| i | M | 26 weeks | 4.9 | 26.1 | —- | —- | E | M |
| j | M | 26 weeks | 21.9 | 30.7 | —- | —- | E | M |
Lungs from SARS-CoV-2 infected mice (M#2–4, F#11–13) that were euthanized at day 4 post-infection to estimate lung viral copies by RT-qPCR. Lungs from uninfected mice a-j were pooled to assess human ACE2 mRNA and protein expression.
^A: A02:01/A24:02/B13:02/B44:02/DR01:01/DR04:01.
^B: A01:01/A02:01/B08:01/B15:01/DR03:01/DR04:01.
^C: A02:01/A24:02/B44:02/B52:01/DR04:01/DR11:04.
^D: A02:01/A02:01/B18:01/B44:02/DR04:01/DR11:04.
^E: 02:01/A02:01/B08:01/B27:05/DR03:01/DR04:01.