Figure 3.

Inhibition of the binding interaction between the α7-AChR and α-cobratoxin using IgGs. (a) schematic representation of a receptor blocking assay, wherein the ability of IgG antibodies to inhibit the interaction between the α7 subunit of AChR (α7-AChR) and α-cobratoxin can be quantified. (b) antibodies block α-cobratoxin binding to its receptor (α7-AChR) in a concentration-dependent manner. as a negative control an IgG specific to dendrotoxins was used and showed no blocking (data not shown). Y-axis is signal relative to that of the positive control.