Table 1.
Primer sequences in RT-PCR
| Forward | Reverse | |
|---|---|---|
| GAPDH | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
| IFN-γ | CCAAGTTTGAGGTCAACAACCC | CGAATCAGCAGCGACTCCTT |
| TNF-α | TCCCAGGTTCTCTTCAAGG | CTGGTATGAGATAGCAAATCGG |
| IL-12a | TGCCTTGGTAGCATCTATGAGG | CGCAGAGTCTCGCCATTATGAT |
| IL-18 | GACTCTTGCGTCAACTTCAAGG | CAGGCTGTCTTTTGTCAACGA |
| IL-21 | CAGGCTAAGAGCTTGTATCGTTTGG | AGGACTGGCTGAGTCTTGAGCAC |
| IRF-1 | GTTGTGCCATGAACTCCCTG | GTGTCCGGGCTAACATCTCC |
| CD3 | CCTGAAAGCTCGAGTGTGTGAGTA | GATGGGCTCATAGTCTGGGTTG |
| B220 | GTTATCCACGCTGCTGCCTCAC | TTGGCTGCTGAATGTCTGAGTGTC |
| F4/80 | TGACTCACCTTGTGGTCCTAA | CTTCCCAGAATCCAGTCTTTCC |
| ROR-γt | GCCGCGGAGCAGACACACTT | GGAGGCCCCCTGGACCTCTG |
| Foxp3 | CAGGAGAAAGCGGATACCAAATG | ATCTGTGAGGACTACCGAGCC |
| IL-17 | TCAGCGTGTCCAAACACTGAG | CGCCAAGGGAGTTAAAGACTT |
| IL-6 | ACAACCACGGCCTTCCCTACTT | CACGATTTCCCAGAGAACATGTG |