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Changes in the OD660 (A) and glucose and CO2 levels (B) in the batch cultures of the wild type (WT) and the pyk mutant (MT) of B. subtilis with an initial glucose concentration of about 8 g/liter. CO2/N2 is the molar ratio of CO2 to N2 in the effluent gas stream. Since the amount of N2 does not change, the variation in the CO2/N2 ratio represents the changes in the CO2 concentration.
