TABLE 1.

Yield and acid production in cultures of the B. subtilis pyk mutant (pyk MT) and the wild type (WT)^a

Trial	Cell type	Amt (g/liter) of:					% Carbon in:
		Glucose in medium	Residual glucose	Acetate	Pyruvate	Lactate	Cells	Acids	CO2
1	pyk MT	4.4 ± 0.2	0	0	0	0	55	0	64
	WT	4.4 ± 0.2	0	0.68 ± 0.11	0	0	47	15	43
2	pyk MT	8.2 ± 0.3	3.4	0	0	0	44	0	74
	WT	7.8 ± 0.3	0	1.16 ± 0.18	0.66 ± 0.08	0.93 ± 0.12	36	37	36

^aPercent carbon in cells is defined as (grams of carbon in cells/total grams of carbon consumed) × 100. The amount of carbon consumed is calculated based on the initial and final glucose concentrations. Percent carbon in acids and that in CO₂ are based on grams of carbon in either acids or CO₂/total grams of carbon consumed. The acids and glucose concentrations were measured three times, and the standard deviations are relatively small, as shown.