TABLE 1.
Stability parameters for soluble compounds in the HS and LS sets of reactors
| Parameter | Reactors | Glucose | Lactate | Butyrate | Acetate | Propionate |
|---|---|---|---|---|---|---|
| Resistance (% of ee of total glucose added) | HS-6, -7, -8 | 2.6, 6.6, 3.7 | 29, 37, 28 | 14, 21, 26 | 5.7, 5.1, 5.0 | |
| HS mean ± SD | (4.3 ± 2.1) | 31 ± 4.9 | 20 ± 6.0 | 5.3 ± 0.38 | ||
| LS-1, -2, -3 | 68, 60, 58 | 53, 58, 50 | 34, 31, 33 | 5.5, 4.1, 8.4 | ||
| LS mean ± SD | 62 ± 5.3 (0.003)a | 54 ± 4.0 (0.009) | 33 ± 1.5 (0.075) | 6.0 ± 2.2 (0.626) | ||
| Resilience (days) | HS-6, -7, -8 | 0.63, 0.83, 0.83 | 0.65, 0.92, 0.92 | 8, 10, 10 | 13, 16, 14 | 16, 20, 18 |
| HS mean ± SD | 0.76 ± 0.12 (0.010) | 0.83 ± 0.16 | 9.3 ± 1.2 | 14 ± 1.5 | 18 ± 2.0 | |
| LS-1, -2, -3 | 0.25, 0.38, 0.33 | 0.26, 0.42, 0.42 | 16, 16, 16 | 26, 24, 26 | 32, 35, 35 | |
| LS mean ± SD | 0.32 ± 0.07 | 0.37 ± 0.09 (0.021) | 16 ± 0.0 (0.010) | 25 ± 1.2 (0.002) | 34 ± 1.7 (0.002) | |
| Reactivity (% ee · day−1) | HS-6, -7, -8 | 12, 23, 16 | 71, 102, 81 | 31, 27, 32 | 2.7, 2.2, 1.7 | |
| HS mean ± SD | 17 ± 5.6 | 85 ± 16 | 30 ± 2.7 | 2.2 ± 0.50 | ||
| LS-1, -2, -3 | 256, 221, 181 | 41, 42, 52 | 3.1, 2.0, 2.0 | 4.5, 1.6, 12 | ||
| LS mean ± SD | 219 ± 38 (0.012) | 45 ± 6.1 (0.056) | 2.4 ± 0.64 (0.003) | 6.0 ± 5.4 (0.343) | ||
| Declivity (% ee · day−1) | HS-6, -7, -8 | 155, 120, 120 | 1.9, 6.0, 3.4 | 4.2, 4.1, 3.2 | 1.2, 1.4, 2.0 | 0.95, 0.85, 0.50 |
| HS mean ± SD | 132 ± 20 | 3.8 ± 2.1 | 3.8 ± 0.55 | 1.5 ± 0.42 | 0.77 ± 0.24 | |
| LS-1, -2, -3 | 385, 240, 240 | 39, 38, 37 | 3.8, 4.1, 3.4 | 2.6, 2.6, 3.0 | 0.92, 0.32, 0.76 | |
| LS mean ± SD | 288 ± 84 (0.088) | 38 ± 1.0 (0.002) | 3.8 ± 0.35 (0.871) | 2.7 ± 0.23 (0.022) | 0.67 ± 0.31 (0.687) | |
| Stability (% ee · day2)b | HS-6, -7, -8 | 12, 22, 23 | 1.1, 6.6, 3.0 | 383, 819, 617 | 1,078, 1,424, 1,118 | 406, 552, 540 |
| HS mean ± SD | 19 ± 6.1 | 3.6 ± 2.8 | 606 ± 218 | 1,207 ± 189 | 499 ± 81 | |
| LS-1, -2, -3 | 5.3, 5.3, 3.0 | 42, 55, 25 | 1,412, 1,750, 1,544 | 5,981, 4,825, 5,863 | 2,111, 1,819, 4,446 | |
| LS mean ± SD | 4.5 ± 1.3 (0.057) | 41 ± 15 (0.052) | 1,569 ± 170 (0.009) | 5,556 ± 636 (0.008) | 2,792 ± 1,440 (0.110) |
a
The values in parentheses are the P values for 95% confidence intervals for two-sample t tests conducted on the corresponding stability parameters for the two reactor sets. A P value less than 0.05 implies that the difference in the means for the two sets was significant at the 95% confidence interval.
b
The total soluble compound stability for the HS set was 2,335% ± 473% ee · day2, and the total soluble compound stability for the LS set was 9,962% ± 1,750% ee · day2 (P = 0.018).