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Triptolide aggravated ROS accumulation via inhibiting Nrf2/HO-1 pathway. (a, b) ROS levels were assessed by flow cytometry using DCFH-DA probe. (c) The protein levels of SLC7A11 and GPX4 were measured by Western blots. (d, e) The quantitative analysis of the indicated proteins. ns: no significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 vs. the control group.
