Figure 6.

Fer-1 attenuates TIC by restoring SLC7A11/GPX4 axis. (a–d) AC16 cells were pretreated with or without fer-1 (2 μM) for 1 h and then exposed to triptolide (30 nM) for another 24 h. (a) Cell viability of AC16 cells were detected using CCK8 assays. (b) MDA levels were detected by commercial assay kits. (c) Reduced GSH levels in AC16 cells. (d) The protein levels of SLC7A11 and GPX4 were measured by Western blots. (e, f) The quantitative analysis of the indicated proteins. ^∗∗∗∗p < 0.0001 vs. the control group, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 vs. the triptolide group.