Figure 3.

ADSC-Exos promote spermatogenic cell proliferation and inhibit apoptosis in vivo. (a) The expression of Ki67 was detected by immunohistochemistry at days 3 (n = 6) and 7 (n = 6). (b) TUNEL assay of testicular tissues after torsion-detorsion injury at days 3 (n = 6) and 7 (n = 6). (c) The expression of Cleaved Caspase-3 was detected by immunofluorescence at days 3 (n = 6) and 7 (n = 6). (d) Quantitative analysis of Ki67, TUNEL, and Cleaved Caspase-3 expression. Bars, 50 μm. Data are represented as mean ± SD. ∗∗∗P < 0.001, ∗∗∗∗P < 0.001.