Figure 7.

Silibinin decreased reactive oxygen species (ROS) content in FA-induced mouse brains. ROS were labeled using DHE (red). The higher red fluorescence intensity per field represents greater ROS content. Fluorescence intensities of DHE in the hippocampal CA1 (a) and cortex (b) are shown. Scale bars represent 100 μm. Quantifications of the fluorescence intensity of DHE are shown in (c) and (d). The data are presented as the mean ± SEM and were analyzed with one-way ANOVA, N = 5 animals per group, ##p < 0.01 compared with the sham group; ^∗p < 0.05 and ^∗∗p < 0.01 compared with the FA group.