Table 2.
Diagnostic results and rpoB, katG, and inhA gene mutation with MDR-TB drug-resistance pattern in Mycobacterium tuberculosis
| 1st author name | Conventional phenotypic DST | Molecular diagnostic tool(s) | Genotypic DST (molecular assay) | rpoB, katG, inhA gene polymorphism | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total isolates with DST test (n) | Undetected (n) | Susceptible for all drugs (n) | Any drug-resistance (n) | INHR (n) | RIFR (n) | MDR (n) | INHR (n) | RIFR (n) | MDR (n) | |||
| Yigzaw et al. [22] | 126 | NR | 83 | 43 | 25 | 12 | 6 | GenoType® MTBDRplus v2.0 | 17 | 3 | NR | rpoB WT2 (1), rpoB WT8 S450L (S431L) (1), rpoB WT8 L452P (L533P) (1) katG S315T1 (12) inhA C15T (7) |
| Ayalew et al. [23] | NS | NS | NS | NS | NS | NS | NS | GenoType MTBDRplus VER 2.0. | 6 | 2 | 2 | rpoB: S531L (2), katG: S315T1 (3), S315T2 (1) inhA: C15T (1), unknown-inhA MUT1 (1) |
| Tilahun et al. [24] | NS | NS | NS | NS | NS | NS | NS | GenoType MTBDRplus | 13 | 2 | 2 | rpoB: S531L (1), WT7/MUT2A (H526Y) (1) katG: S315T1 (10) inhA: C15T (4) missed, WT1+MUT1 (katG + inhA (2) |
| Welekidan et al. [25] | NS | NS | NS | NS | NS | NS | NS | GenoType MTBDRplus and MTBDRsl | 41 | 40 | 38 | rpoB: S531L (28), H526Y (4), H526D (3), D516V (2), unknown – poB WT3 (2), rpoB WT4 (1), rpoB WT6 (1), rpoB WT8 (2), rpoB WT7 (2), katG: S315T (32), unknown- katG WT (7) inhA: C15T (2), unknown- inhA WT2 (1) |
| Gashaw et al. [26] | 69 | 4 | 54 | 11 | 7 | 6 | 5 | GenoTypic MTBDRplus assay | 16 | 14 | 8 | rpoBWT8 missed (10), WT3 (1), WT4 (1), WT6 (1), WT7 (1) katGWT missed (8), inhAWT1 (-15/-16) (5) |
| Welekidan et al. [27] | NS | NS | NS | NS | NS | NS | NS | Whole-genome sequencing (WGS) | 26 | 25 | 23 | rpoB: S450L (18), H445Y (1), H445D (1), D435V (1), H445N (2), D435Y (1), L430P (1) katG: S315T (25) |
Note. NS, not studied; NR, not reported.