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. 2022 Jun 22;221(8):e202103207. doi: 10.1083/jcb.202103207

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© 2022 Georgantzoglou et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — Utrophin marker distribution during neutrophil gradient responses. In support of Fig. 3. (A) Time-lapse sequence of two-photon confocal image projections showing a neutrophil (white) in a Tg(mpx:Lifeact-Ruby) zebrafish larva (top row), injected with GFP-UtrCH (bottom row), migrating pre- and post-LW. White arrows indicate the speed vector. Red/green arrows indicate the side of cell with higher Lifeact/Utrophin abundance. Scale bar = 10 μm. (B and C) Neutrophil Lifeact and Utrophin polarity (front/rear) in relation to time; Lifeact-Ruby is shown in red and Utrophin is shown in green. Time sequence was synchronized based on time of the LW (B) or the time that each individual neutrophil beginning of movement post-LW (C). n = 15 cells from 13 larvae.