Table 1.
Gene-specific primer sequence and RT-PCR parameter.
| Gene | Primer sequence (5′–3′) | AT (°C) |
|---|---|---|
| IRS-1 |
TCCCCCTGCCCAAGGATATT (F) GAAAGGCAGTGGGTCTAGGG (R) |
58 |
| GLUT-4 |
CAACGTGGCTGGGTAGGCA (F) ACAACATCAGCCCAGCCGGT (R) |
58 |
| DPP-IV |
GAGCTAATAACACCACTGAGGCAT (F) GAGCAAAAGTAGTACGTGCCAAG (R) |
53 |
| GLP-1R |
CATCCACCTGAACC5GTTTGC (F) GGGCAGCGTCTTTGATGAA (R) |
56 |
| AKT |
CCGCTATTATGCCATGAAGAT (F) TGTGGGCGACTTCATCCT (R) |
54 |
| PI3K |
CAAAGCCGAGAACCTATTGC (F) GGTGGCAGTCTTGTTGATGA (R) |
54 |
| GAPDH |
CTGACATGCCGCCTGAAAC (F) CCAGCATCAAAGGTGGAAGAA (R) |
51 |
AT, annealing temperature.