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. 2022 Jun 23;13:3588. doi: 10.1038/s41467-022-31364-7

Fig. 3. hnRNPH1 is indispensable for embryonic development and male fertility in mice.

Fig. 3

a Representative embryos at various developmental time points, including embryonic day 9.5 (E9.5), E10.5, E12.5, E13.5, and E14.5, as derived from control and hnRNPH1−/− mice. b Real-time qPCR analyses of Hnrnph1 and Hnrnpf mRNA levels in control and hnRNPH1 cKO testes of adult mice. Data were presented as mean ± SD, n = 3 (three biological replicates). A two-sided Student’s t-test was performed, ***p = 0.0005. c Western blotting analyses of hnRNPH1 and hnRNPF protein in control and hnRNPH1 cKO testes of adult mice. GAPDH was used as a loading control. d Immunofluorescence (IF) staining of hnRNPH1 in control and hnRNPH1 cKO testes of adult mice. DDX4 was co-stained to indicate the location of the germ cell. The DNA was stained with DAPI. Scale bars = 50 μm. e–f Co-immunofluorescence staining of PTBP2 (e) and SRSF3 (f) with SYCP3 in testis sections from adult control and hnRNPH1 cKO mice. The DNA was stained with DAPI. Scale bars = 50 µm. g Gross morphology of the testes and the epididymides from adult control and hnRNPH1 cKO mice. h Testis growth curves of control and hnRNPH1 cKO mice from postnatal day 7 (P7) to P56. Data were presented as mean ± SD, n = 3 (three biological replicates). A two-sided Student’s t-test was performed, left to right: **p = 0.0089, ***p = 0.0009, ***p = 0.0004, ***p = 0.0001. i Periodic acid-Schiff (PAS) staining of testes and epididymides from control and hnRNPH1 cKO mice at P56. Scale bars = 50 μm. j Co-immunofluorescent staining of DDX4 with SYCP3 on control and hnRNPH1 cKO cauda epididymidis sections at P56 and P24, respectively. Scale bars = 50 μm. Biologically independent mice (n = 3) were examined in three separate experiments (c–f, i, j). Source data are provided as a source data file.