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. 2022 Jun 23;13:3588. doi: 10.1038/s41467-022-31364-7

Fig. 6. Ablation of hnRNPH1 in germ cells results in abnormal AS of cell junction-related genes and defective germ-Sertoli cell communications.

Fig. 6

a Representative examples of RT-PCR analyses for indicated AS events differentially regulated genes between control and hnRNPH1 cKO round spermatids are shown. Middle panels represent the schematic diagram of alternatively spliced exons detected by RNA-Seq analysis. Right panels show the quantification of percent spliced in (PSI). Data were presented as mean ± SD, n = 3 (three biological replicates). A two-sided Student’s t-test was performed. b Western blotting analyses of the expression of TCF7L1, TCF7L2, TCF3, IQSEC1, FNDC3A, RAPGEF6, and hnRNPH1 in control and hnRNPH1 cKO round spermatids isolated from P25 mice. GAPDH was used as a loading control. c–e Co-immunofluorescence staining of SYCP3 with TCF7L1 (c), TCF7L2 (d), and β-catenin (e) on testis sections from control and hnRNPH1 cKO mice at P56. Scale bars = 50 µm. f Fluorescence microscopy to detect F-ACTIN (phalloidin) and DNA (DAPI) in testes from control and hnRNPH1 cKO mice at P56 (left panels) and P24 (right panels). Scale bars = 50 µm. Biologically independent mice (n = 3) were examined in three separate experiments (b–f).