Fig. 3.

In vitro role of FMOD in tumor angiogenesis. Sub-culture of human endothelial cells (HUVECs) with either conditioned medium from Cl.2A cells (Control vs FMOD−/−) (A) or recombinant human FMOD (B) has no effect on HUVECs proliferation. C Transmigration potential of HUVECs across a fibronectin-coated barrier toward Cl.2A cells is significantly reduced upon the loss of FMOD. D Representative images of HUVECs tube formation following treatment with either recombinant human FMOD or VEGF (Scale 100 µm). Quantification and analysis of the number of branches (E), junctions (F), meshes (G), and the total tube length (H) of HUVECs following treatment with either recombinant FMOD or VEGF. All data are expressed as mean ± SD of three biological replicates and statistical significance is determined using a two-sided Student’s t test: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001