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Construction of the streptococcal gfp vectors pMA2 and pMA3. Promoterless gfp incorporating SacI and BamHI sites was PCR amplified from pBJ169 (a) and subcloned into pMH109-HPP (b) downstream of PhppA to generate pMA1 (c). PhppA-′gfp incorporating BamHI sites was PCR amplified from pMA1 and subcloned into the BamHI site of pDL277 (d) to generate pMA2 (e) and into the BamHI site of pVA838 (f) to generate pMA3 (g).
