TABLE 2.
List of plasmids used
| Plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| pMH109 | Tcr Kmr ′cat cassette; Streptococcus-E. coli shuttle vector | 18 |
| pBJ169 | Apr; ′gfp cassette | B. Julien and D. Kaiser |
| pDL277 | Spr; Streptococcus-E. coli shuttle vector | 30 |
| pVA838 | Emr Cmr; Streptococcus-E. coli shuttle vector | 34 |
| pMH109-HPP | Tcr Kmr Cmr; pMH109 containing PhppA-′cat cassette; Streptococcus-E. coli shuttle vector | This study |
| pSF143 | Tcr; Streptococcus integration vector | 51 |
| pSFCAT2 | Tcr Cmr; pSF143 containing PhppA-′cat cassette | This study |
| pMA1 | pMH109-HPP containing an 0.8-kb SacI-BamHI ′gfp cassette in place of the ′cat cassette | This study |
| pMA2 | pDL277 containing a 1.2-kb BamHI-BamHI PhppA-′gfp cassette | This study |
| pMA3 | pVA838 containing a 1.2-kb BamHI-BamHI PhppA-′gfp cassette | This study |
a
Resistance phenotypes: Ap, ampicillin; Sp, Spectinomycin; Em, Erythromycin; Km, kanamycin; Cm, chloramphenicol; Tc, tetracycline.