Table 3.
NECTIN4 protein expression in multifocal urothelial HR NMIBC patients
| Overall staining pattern | Membranous staining | |||||
|---|---|---|---|---|---|---|
| Entity (Patients) |
Positive N (%) | Negative N (%) | Heterog N (%) |
Positive N (%) | Negative N (%) | Heterog N (%) |
| CIS/T1HG (N = 47) | 43 (91.5) | 0 | 4 (8.5) | 42 (89.4) | 4 (8.5) | 1 (2.1) |
| Pure TaHG/T1HG (N = 22) | 21 (95.5) | 0 | 1 (4.5) | 19 (86.4) | 1 (4.5) | 2 (9.1) |
|
Mixed TaLG/HG (N = 18) |
12 (66.7) | 2 (11.1) | 4 (22.2) | 9 (50.0) | 3 (16.7) | 6 (33.3) |
Intra-entity-specific heterogeneity of NECTIN4 protein expression in different HG NMIBC subentities was considered by analyzing independent tumor biopsies of the same HG subentity (range: 2–7 (CIS), 2–6 (pure TaHG/T1HG), 2–3 (mixed TaLG/HG)) taken from different locations in the same urinary bladder, respectively. For multifocal mixed TaLG/HG tumors, only staining of the HG portion was considered. Heterog: independent biopsies of the same patient show heterogenous (i.e., negative and positive) NECTIN4 protein expression (based on H-score [9])