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. 2022 Jun 23;13:3586. doi: 10.1038/s41467-022-31206-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Surface expression of 3HA-tagged F508del-CFTR in BHK cells incubated for 24 h with 10 μM compound and treated with mouse monoclonal anti-HA antibody and anti-mouse IgG conjugated with FITC (n = 4 or n = 3 biological replicates, bars represent the mean). b, c FLIPR Membrane Potential (FMP) assay of F508del-CFTR expressing BHK cells treated for 24 h with the macrocycles (b, n = 6 biological replicates, bars represent the mean) or with CY007424 and/or VX-809 or Trikafta®, with and without CFTR inhibitor INH172 (c, n = 4 biological replicates, bars represent the mean). d Using chamber experiment of F508del-CFTR expressing CFBE cells treated for 18 h with CY007424, VX-809, or Trikafta® or in combinations. CFTR activity is assayed by measurement of short-circuit current of cells after stimulation by forskolin and the potentiator genistein (n = 3 or n = 6 biological replicates, bars represent the mean). Source data are provided as a Source Data file.