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. 2022 Jun 23;13(6):566. doi: 10.1038/s41419-022-05015-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Surgical lung-cancer samples were collected and divided into tumor and para-tumor region, P, para tumor, T, tumor, ASNS were detected in protein level (A) and mRNA level (B). C in the tissue microarray of lung cancer, ASNS protein was tested by immunohistochemistry among 92 matched samples and scored by the Quantitative Pathology Imaging System (PerkinElmer), ASNS expressed level was analyzed by ANOVA. D 92 patients were divided into high ASNS and low ASNS groups by Immunohistochemical score, and different overall survivals were tested by Log-rank (Mantel–Cox) test. E 92 patients were divided into metastasis and non-metastasis groups, the significance of different ASNS levels was tested by Student’s t test. F 1706 samples were collected from TCGA and grouped by metastasis and non-metastasis, ASNS mRNA level was analyzed by Student’s t test, data download from https://xenabrowser.net/datapages/. G ASNS protein sequence was annotated, amino acid mutations were also shown on ASNS domains. H, I survival of different ASNS mutated groups were analyzed by Kaplan–Meier survival analysis. Error bars were expressed as mean ± S.D. P < 0.05 is significant statistical difference, *p < 0.05, **p < 0.01, ***p < 0.001. Representative images from three independent experiments are shown.