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. 2022 Jun 23;13(6):566. doi: 10.1038/s41419-022-05015-0

Fig. 3. ASNS stabilizes the β-catenin complex and upregulates mitochondrial potential.

Fig. 3

The whole-cell RNA was extracted and put into RNA sequencing (A). Some pathways were enriched in the differential gene expression pool (B). C RNA sequencing data was collected from the TCGA, then ranked Wnt pathway-related genes by gradient ASNS mRNA level from higher to lower. β-catenin, an important secondary messenger was tested in ASNS overexpressed (D) and knockdown (E) cell lines. F The stability of β-catenin complexes was detected by blue native page in overexpressed and knockdown cell lines. G 11 mitochondrial coding mRNAs and POLG2 mRNA were found in differential expression gene pools. H TMRM was a special probe to detect mitochondrial potential. The mitochondrial potential was detected in overexpressed and knockdown cell lines; the white bar was 20 μm. Error bars were expressed as mean ± S.D. P < 0.05 is significant statistic difference, *p < 0.05, **p < 0.01, ***p < 0.001. Representative images from three independent experiments are shown.