Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 23;13:3583. doi: 10.1038/s41467-022-31185-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Three loss-of-function (LoF) alleles in zebrafish were generated by CRISPR/Cas9 injections targeting smchd1. b Endogenous spatial-temporal expression pattern of smchd1 in zebrafish embryos as determined by qPCR and WISH. n = 3 biological samples (qPCR), 3 independent experiments with at least 20 embryos each (WISH). Data are presented as mean values ± SD. c Maternal zygotic (MZ) smchd1^lof embryos lack smchd1 mRNA (qPCR and WISH) and protein (Western blot), suggestive of total nonsense-mediated decay. Scale bar = 200 μm. n = 2 biological samples (qPCR, Western blot), 3 independent experiments with at least 20 embryos each (WISH). d MZ smchd1^lof zebrafish are viable and fertile, and do not model arhinia at 3 months. Scale bar = 1 mm.