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. 2022 Jun 23;7:61. doi: 10.1038/s41541-022-00483-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Lm-GUCY2C secretes a fusion protein comprised of ActAN100*, an enhancer sequence, and mouse GUCY2C_23-429 under the control of the actA promoter. b, c J774A.1 macrophages were uninfected or infected with Lm-Control or Lm-GUCY2C at a 10:1 MOI for 6 h at 37 °C. GUCY2C fusion protein was detected by (b) western blot and (c) immunofluorescence. Scale bars in (c) are 25 μm. Full western plots are shown in Supplementary Fig. S7.