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. 2022 Jun 23;13:3306. doi: 10.1038/s41467-022-30944-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic representation of SR imaging. The immobilized RRM1-2 aggregate is transiently bound by an Atto590-tagged Apt1 molecule, the position of which is determined with nanometer precision. This process is repeated to generate a SR image of each aggregate. b Example time montage of an oligomer being imaged using PAINT. Each sub-image is separated by 1 s, moving through time from left to right then top to bottom; scale bar: 400 nm. c Intensity profile of the oligomer imaged in (b). Individual localizations appear as bursts in intensity that are separated in space and time. d Histogram of precisions of the oligomer imaged in (b). Each localization is accurate positioned with a precision of 60 nm or less. e SR image (red hot) and diffraction-limited (gray) images of the aggregate shown in (b), scale bar is 500 nm. f Sample fields of view of clustered RRM1-2 aggregates imaged with Apt-1 and nApt-1, scale bar 1 μm. Data from 3 independent experiments. g Compared to Apt-1 nAPt-1 detects significantly less RRM1-2 aggregates. The data shown are mean ± SD of 9 fields of view. ****p = 1.9769 × 10⁻¹¹; analyzed by two-sided t-test, confidence interval 95%. Source data are provided as a Source data file⁶⁴.