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. 2022 Jun 23;13:3306. doi: 10.1038/s41467-022-30944-x

Fig. 7. Confocal microscopy analysis of TDP-43 and nApt-1 in live mammalian cells.

Fig. 7

Blue: DAPI; green: eGFP_TDP-43; red: nApt-1_Atto590. a Wide view of Hek293T cells co-transfect with the plasmid for the overexpression of full-length TDP-43 fused to eGFP and the aptamer nApt-1 conjugated to the fluorophore Atto590. b–d Examples of isolated cells co-transfected as in (a), in which the lack of correlation between green and red fluorescence distribution is evident. e–g Fluorescence profiles of DAPI, eGFP, and Atto590 along a diagonal line drew across the isolated correspondent cells on the left, showing how nApt-1_Atto590 mostly localized in the peri-cytoplasmatic regions, irrespective of the distribution of eGFP_TDP-43.