Fig. 2.

TRPV4 inhibition decreased 4-AP-induced spontaneous EEG seizures and neuronal damage in mice. a The behavior of mice was recorded for 2 h after 4-AP injection. Representative traces of EEG recordings and the corresponding enlarged part in control, 4-AP, 4-AP + HC-067047, and 4-AP + GSK1016790A mice at the middle of the 2 h window (n = 10). b–e Seizure severity (b), seizure latency (c), seizure duration (d), and mortality (e) of mice after 4-AP treatment in the presence or absence of a TRPV4 agonist and antagonist (n = 10). f HC-067047 markedly attenuated 4-AP-induced hippocampal neuronal damage (n = 6). g–i Nissl staining in the hippocampal DG, hilar, and CA3 regions in the 6 groups of mice. The brains were collected 24 h after 4-AP injection. The data are presented as the means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, Tukey’s test after one-way analysis of variance (ANOVA)