Fig. 6.

TRPV4 promoted astrocyte activation and proinflammatory cytokine production via YAP/STAT3 signaling pathway activation. a Representative immunoblots showing the inflammatory signaling pathways in primary cultured astrocytes after the different treatments (n = 3). b, c Quantitative analysis of the protein (b) and mRNA (c) levels of YAP (n = 3). d, e Quantitative analysis of the absolute p-YAP level (normalized to total YAP) (d) and p-STAT3 level (normalized to total STAT3) (e) (n = 3). f Representative confocal images showing that 4-AP or the TRPV4 agonist GSK1016790A increased YAP nuclear localization. The TRPV4 antagonist reversed this effect (n = 3). The data are presented as means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, Tukey’s test after ANOVA