Fig. 3.

Chk1 overexpression induces cognitive deficits in mice. a–n Sixteen mice aged 8 weeks were divided into two groups and injected with AAV-Con and AAV-Chk1virus respectively into the bilateral ventricles. After 4 weeks, all mice were tested by different behavioral test paradigms. a A schematic diagram for the behavioral tests (novel objection recognition test (NOR), fear conditioning test (FCT), Morris water maze test (MWM)) of the mice. b The infection of the virus in the hippocampus was confirmed by fluorescence microscopy. c The total time (s) and distance (m) of moving in an open field test. d The experimental design of novel object recognition test (NOR). Left is the acquisition trial. The test trial was conducted 24 h (right) after the acquisition trial. e Left: the total exploration time (s) to object A and object B in the acquisition trial; right: the total exploration time (s) to object A and object C in the test trial. f Left: the recognition index to object A and object B in the acquisition trial; right: the recognition index to object A and object C in the test trial. g The total freezing time (s) and freezing times of the mice in the context test. h The total freezing time (s) and freezing times of the mice in the altered context and tone test. i The representative searching trace on day 5 of the training and the probe trial at 48 h after training in MWM. j The latency of the mice to find the hidden platform. k–n The time in the target quadrant (s) (k), the percentage of time in the target quadrant (l), the crossing times (m), the swimming speed (n) of the mice. All data represent mean ± SEM, n = 8, *P < 0.05, **P < 0.01, compared to controls