Fig. 7.

Chk1 inhibitor (GDC-0575) rescues neuron loss and synaptic impairments in APP/PS1 mice. a Representative images of Nissl staining of the mice brain slices. b The quantitative analysis of the neuron number in the CA3 region of a. The cell number in the hippocampal CA3 area was counted in 2–3 brain slices from each mouse (N = 3) in each group. c Representative images of Golgi staining of the mice hippocampal slices. d The quantitative analysis of the spine number in c. Spines in 11–17 intact dendrites in the hippocampal DG area were counted in each group. e Representative immunoblots of synaptic proteins (PSD95, Synapsin I, Synaptophysin (SYP), and β-actin) in the hippocampus and cortex of the mice. Blots were from different gels with the same batch of samples electrophoresed. f The quantitative analysis of the protein levels in the hippocampus in e. Bands intensity was normalized to β-actin levels. g The quantitative analysis of the protein levels in brain cortex in e. All data represent mean ± SEM, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001