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. 2022 Jun 10;3:897115. doi: 10.3389/froh.2022.897115

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Copyright © 2022 Sorsa, Nwhator, Sakellari, Grigoriadis, Umeizudike, Brandt, Keskin, Tervahartiala, Pärnänen, Gupta, Mohindra, Bostanci, Buduneli and Räisänen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative Western immunoblot for molecular forms and species of MMP-8/collagenase-2 in human mouthrinse samples. Lane 1: recombinant human MMP-8, polyclonal antibody; lane 2: recombinant human MMP-8 activated by 200 μM NaOCl, polyclonal antibody; lane 3: periodontitis mouthrinse before treatment with polyclonal anti-MMP-8; lane 4: periodontitis mouthrinse after treatment with polyclonal anti-MMP-8; lane 5: periodontitis mouthrinse before treatment with monoclonal anti-MMP-8; lane 6: periodontitis mouthrinse after treatment with monoclonal anti-MMP-8. The p- and aMMP-8 (PMN and Mes) indicate neutrophil pro (p) and active (a) (PMN)- and fibroblast-type (Mes), MMP-8, respectively, and fragments indicate lower (<50 kDa) size MMP-8 species. Molecular weight markers are indicated on the left side.