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. 2022 Jun 23;13:3580. doi: 10.1038/s41467-022-31318-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — (a) Distributions of time-normalized radii of gyration. Mobile cutoff was determined to be 16.9 nm, as indicated by the pink dashed line. (b) Percentage of molecules identified as mobile increases as a function of temperature. (c) Median diffusion coefficients for mobile and immobile molecules as a function of temperature. Dashed line indicates median D_T from 300.0 K baseline data with only positive diffusion coefficients included. (d) Median asymmetry coefficient (a₂) as a function of temperature for molecules identified as mobile and immobile. Values closer to 0 indicate more anisotropic trajectories. (e) Degree of DSE breakdown vs. temperature as obtained from imFCM (navy symbols) and median single molecule results for all (green) and mobile (pink) molecules. Navy line is a polynomial fit to the imFCM data. Black dashed line indicates DSE behavior.