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. 2022 Jun 10;12:876732. doi: 10.3389/fonc.2022.876732

Figure 5.

Figure 5

Comparison of pipelines. (A–C) TaqMan Advanced (global) microRNA pipeline. (D–F) TaqMan (target-specific) microRNA pipeline. (G, H) DigiMir pipeline. The x-axis represents the samples used for the optimization phase: #1 and #2—stage I seminomas; #3—stage II embryonal carcinoma; #4—stage III embryonal carcinoma; #5—age-matched healthy blood donor. The color code refers to experimental settings: setting #1—different extraction; setting #2—different cDNA synthesis; setting #3—different operator; setting #4—same operator; and reference for comparison.