Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 10;50(11):6435–6452. doi: 10.1093/nar/gkac362

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — Schematic illustration of the applied PNA technology and the PNA target region of the conserved essential gene, acpP. (A) An antisense PNA is coupled to a CPP to facilitate intrabacterial delivery. When targeting essential bacterial genes, CPP-PNA conjugates can serve as potent antimicrobial molecules. Parts of this image have been created with Smart (Servier medical art). (B) Multiple sequence alignment (created with http://multalin.toulouse.inra.fr/multalin/; (118)) of the essential gene acpP including the following γ-proteobacteria: UPEC 536 (CP000247.1), E. coli CFT073 (CP051263.1), E. coli K-12 (CP032667.1), Shigella dysenteriae (CP000034.1), Citrobacter rodentium (CP038008.1), Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (AE006468.2), Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 (FQ312003.1), Klebsiella pneumoniae (CP003200.1) and Yersinia pestis (NC_003143.1). A defined section (–40 to +90 nt) including the region around the PNA binding site (grey box) is shown. The following color-code has been applied: perfect consensus black, varying alignment columns cyan (nucleotide substitution green). (C) Relevant region of acpP mRNA in UPEC 536, with the PNA target sequence shaded in green. The start codon (AUG) is shown in bold type. Below, the PNA sequence is shown (green box) with the different conjugated CPPs for delivery into UPEC ((KFF)₃K, (RXR)₄XB, Tat, Dap9).