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. 2022 Jun 7;50(11):6587–6600. doi: 10.1093/nar/gkac476

Figure 2.

Figure 2.

Characterization of single-input-multi-output genetic circuits. (A) Illustration of the single-input-multi-output genetic circuits. The CFP, GFP and mCherry modules were driven by the temperature-responsive promoters Pveg32 (with transition point at 32°C), Pveg34 (with transition point at 34°C) and Pveg37 (with transition point at 37°C), respectively. Depending on the temperature, the fermentation process was divided into four stages. Three modules were not expressed at 30°C during stage I, and the CFP module was up-regulated at 32°C during stage II. Both of CFP and GFP modules were expressed at 34°C during stage III, and three modules were activated at 37°C during stage IV. (B) Confocal images of the WY10 strains at different fermentation periods from the cyan channel (465–480 nm), green channel (515–564 nm) and red channel (610–669 nm). The merged images from both channels.