Figure 7.

Production of 2′-fucosyllactose in a 5-l bioreactor under ordered control from 30 to 34 to 37°C (changing the temperature to 34 and 37°C orderly after 12- and 18-h growth). (A) Production of 2′-fucosyllactose by MT17 in a 5-l bioreactor under ordered control from 30 to 34 to 37°C. Trends of cell growth, sucrose, lactose, and 2′-fucosyllactose titer during pulse fed-batch fermentation in a 5-l bioreactor (n = 2 biologically independent samples). (B) Production of the 2′-fucosyllactose by MT22 in a 5-l bioreactor by using ordered control. Trends of cell growth, sucrose, lactose, and 2′-fucosyllactose titer during pulse fed-batch fermentation in a 5-l bioreactor (n = 2 biologically independent samples).