Table 1.
Strains used in this study
| Strain | Characteristics | Ref. |
|---|---|---|
| E. coli JM109 | recA1, endA1, thi, gyrA96, supE44, hsdR17Δ (lac-proAB)/F’[traD36,proAB+, laclq, lacZΔ M15] | Lab stock |
| B. subtilis 168 | trpC2 | Lab stock |
| WY1 | B. subtilis 168, ΔamyE:: P43-CI857 | This work |
| WY2 | WY1, pHTa1-PR-GFP | This work |
| WY3 | WY1, pHTa1-2-Pveg-GFP | This work |
| WY4 | WY1, pHTa2-Pveg1-GFP | This work |
| WY5 | WY1, pHTa3-Pveg2-GFP | This work |
| WY6 | WY1, pHTa4-Pveg3-GFP | This work |
| WY7 | WY1, pHTa5-Pveg4-GFP | This work |
| WY8 | WY1, pHTa6-Pveg5-GFP | This work |
| WY9 | B. subtilis 168, pHTa6-Pveg5-GFP | This work |
| WY10 | WY1, ΔnprE:: Pveg32-CFP, ΔganA:: Pveg34-GFP-Pveg37-mCherry | This work |
| WY11 | WY1, pP43-mCherry | This work |
| WY12 | B. subtilis 168, ΔnprE:: Pveg5-dCpf1, ΔganA:: crRNAmCherry, pP43-mCherry | This work |
| WY13 | WY1, ΔnprE:: Pveg5-dCpf1, ΔganA:: crRNAmCherry, pP43-mCherry | This work |
| WY14 | WY8, ΔnprE:: Pveg5-dCpf1, ΔganA:: crRNAmCherry, pP43-mCherry | This work |
| MT0 | WY1, ΔnprE:: P43-manC-gmd-T-P43-wcaG | This work |
| MT1 | MT0, Δbpr:: P43-manB-T-P43-futC | This work |
| MT2 | MT0, Δbpr:: P43-manB-T-Pveg34-futC | This work |
| MT3 | MT0, Δbpr:: Pveg34-manB-T-Pveg34-futC | This work |
| MT4 | MT3, ΔganA | This work |
| MT5 | MT4, ΔyesZ | This work |
| MT6 | MT3, ΔganA:: Pveg-dCpf1 | This work |
| MT7 | MT6, Δepr:: Pveg5-crRNAzwf1- crRNApfkA1 | This work |
| MT8 | MT6, Δepr:: Pveg5-crRNAzwf1- crRNApfkA2 | This work |
| MT9 | MT6, Δepr:: Pveg5-crRNAzwf1- crRNApfkA3 | This work |
| MT10 | MT6, Δepr:: Pveg5-crRNAzwf2- crRNApfkA1 | This work |
| MT11 | MT6, Δepr:: Pveg5-crRNAzwf2- crRNApfkA2 | This work |
| MT12 | MT6, Δepr:: Pveg5-crRNAzwf2- crRNApfkA3 | This work |
| MT13 | MT6, Δepr:: Pveg5-crRNAzwf3- crRNApfkA1 | This work |
| MT14 | MT6, Δepr:: Pveg5-crRNAzwf3- crRNApfkA2 | This work |
| MT15 | MT6, Δepr:: Pveg5-crRNAzwf3- crRNApfkA3 | This work |
| MT16 | MT9, Δldh:: Pveg34-ndk | This work |
| MT17 | MT16, Δldh:: Pveg34-ndk-gmk | This work |
| MT18 | MT17, Δldh:: Pveg34-ndk-gmk-xpf | This work |
| MT19 | MT17, ΔlytC | This work |
| MT20 | MT17, Δapre:: Pveg5-crRNAlytC1 | This work |
| MT21 | MT17, Δapre:: Pveg5-crRNAlytC2 | This work |
| MT22 | MT17, Δapre:: Pveg5-crRNAlytC3 | This work |