Figure 3.
Neural lesions in adult brains caused by perturbation of proper regulation of tj-TR. (A–E) Young (3–4 day old) adult brains stained with DE-Cadherin (Cad). (A, B) Cad staining usually appears homogeneous in control brains (A; e.g. green arrowheads) and is disrupted by instances of sporadic lesions in tjhypo brains (B; red arrowheads). (C–E) tj-TR mutant brain phenotypes. tjnat/nat brains appear similar to control brains (C). Lesions occur sporadically throughout tjTR/TR (D) and tjnTR/nTR (E) brains, similar to tjhypo. Scale bar: 100 μm. (F, G) Quantifications show the percentage of CBs (F) and OLs (G) that exhibit neural lesions, out of the total brains analyzed. At least three biological replicates were performed. n ≥ 42 CBs and n ≥ 74 OLs per genotype. Student's t-test was used to determine significance (*P < 0.05; n.s., not significantly different from control).