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. 2022 Jun 1;50(11):6511–6520. doi: 10.1093/nar/gkac443

Figure 5.

Figure 5.

Binding of miR-142-3p to HSUR1 is independent of the binding of miR-27. (A) Same as in Figure 3 on extracts prepared from HeLa-H1 cells transiently cotransfected with a plasmid expressing miR-27b, miR-142-3p, and Control LNA inhibitor or an LNA inhibitor with complementarity to miR-27. (B) Same as in (A) on extracts prepared from equal number of HeLa-H1 cells or HeLa cells constitutively expressing a mutant version of HSUR1 that is unable to bind miR-27 (HeLa-H1Δ27) transiently cotransfected with a plasmid expressing miR-27b and miR-142-3p. Partial sequences of HSUR1 and miR-27 are shown, with residues involved in basepairing highlighted in grey. Mutated residues are shown in bold.