Figure 4.

Simultaneous conversion of multiple bases by Bi-PE. (A) Schematic diagram showing the design and the putative editing process of Bi-PE-2, Bi-PE-3, L-and R-PE3 strategies. Bi-PE-2 generates a pair of 3′flaps containing only the targeted edits, and Bi-PE-3 generates 3′ flaps containing both edits and HAs humongous to the sequences flanking the editing region. (B) HTS quantifying the frequencies of alleles containing simultaneous conversions of multiple bases. All alleles observed with frequency ≥0.01% were shown. Values and error bars reflect mean ± s.d. of n = 3 independent biological replicates. Data of each replicate were shown in Supplementary Table S5. (C) Summary of multiple bases conversion efficiencies across five endogenous sites in HEK293T cells. The mean of all individual values of n = 3 independent biological replicates was plotted.