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. 2022 Jun 10;13:926262. doi: 10.3389/fimmu.2022.926262

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Copyright © 2022 Isaacs, Amarilla, Aguado, Modhiran, Albornoz, Baradar, McMillan, Choo, Idris, Supramaniam, McMillan, Muller, Young, Woodruff, Wolvetang, Chappell and Watterson

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of SARS-CoV-2 variants by N-specific nanobodies. (A) C2 and E2 nanobodies were formatted as dimeric Fc fusion proteins for ease of purification and detection. Coomassie stained SDS-PAGE shows highly pure products. (B) Differential detection of viral antigens in fixed cell-based ELISA against infected Vero E6 cells. Assays were conducted twice with n = 2. Bmax values with standard error are shown for each variant as calculated by Prism Graphpad 9 software. (C) Detection of viral antigens in infected cell lysates in western blots ran under reducing (+DTT) or non-reducing conditions. Housekeeping protein GADPH was used as a positive control. Molecular weights are shown to the right in kDa.